Insights into Diterpene Cyclization from Structure of Bifunctional Abietadiene Synthase from Abies grandis
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چکیده
منابع مشابه
Insights into diterpene cyclization from the structure of the bifunctional abietadiene synthase from Abies grandis*
Insights into diterpene cyclization from the structure of the bifunctional abietadiene synthase from Abies grandis* Ke Zhou, Yang Gao, Julie A. Hoy, Francis M. Mann, Richard B. Honzatko and Reuben J. Peters From the Department of Biochemistry, Biophysics, & Molecular Biology, Iowa State University, Ames, IA 50011 USA Running title: Structure of abietadiene synthase Address correspondence to: Re...
متن کاملA single residue change leads to a hydroxylated product from the class II diterpene cyclization catalyzed by abietadiene synthase.
Class II diterpene cyclases catalyze bicyclization of geranylgeranyl diphosphate. While this reaction typically is terminated via methyl deprotonation to yield copalyl diphosphate, in rare cases hydroxylated bicycles are produced instead. Abietadiene synthase is a bifunctional diterpene cyclase that usually produces a copalyl diphosphate intermediate. Here it is shown that substitution of aspar...
متن کاملThe primary diterpene synthase products of Picea abies levopimaradiene/abietadiene synthase (PaLAS) are epimers of a thermally unstable diterpenol.
The levopimaradiene/abietadiene synthase from Norway spruce (Picea abies; PaLAS) has previously been reported to produce a mixture of four diterpene hydrocarbons when incubated with geranylgeranyl diphosphate as the substrate: levopimaradiene, abietadiene, neoabietadiene, and palustradiene. However, variability in the assay products observed by GC-MS of this and orthologous conifer diterpene sy...
متن کاملBifunctional cis-abienol synthase from Abies balsamea discovered by transcriptome sequencing and its implications for diterpenoid fragrance production.
The labdanoid diterpene alcohol cis-abienol is a major component of the aromatic oleoresin of balsam fir (Abies balsamea) and serves as a valuable bioproduct material for the fragrance industry. Using high-throughput 454 transcriptome sequencing and metabolite profiling of balsam fir bark tissue, we identified candidate diterpene synthase sequences for full-length cDNA cloning and functional ch...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2012
ISSN: 0021-9258
DOI: 10.1074/jbc.m111.337592